Spin the sample within the tube for 10 minutes at 2500 rpm in a centrifuge. Alternatively, filter the contents from the tube right into a clean up tube applying cheesecloth in addition to a funnel. Touchdown PCR: Annealing temperature over the first two cycles of amplification is about at around https://www.facebook.com/NewTechFind/posts/pfbid022JsHDTLNuxpfdSMfWcoohiK8mMZuFm1P7XpUjVw6pr6yGtPN2whP6BF4p7i2CgFHl